
펙틴 탈메틸화 상태가 RALF1 펩타이드의 FER 수용체 인지와 신호 전달에 필수적이다.


coremsg

The extracellular matrix plays an integrative role in cellular responses in plants, but its contribution to the signalling of extracellular ligands largely remains to be explored. RAPID ALKALINIZATION FACTORs (RALFs) are extracellular peptide hormones that play pivotal roles in various physiological processes. Here, we address a crucial connection between the demethylation machinery of the cell wall component pectin and RALF1 activity. Pectin is a polysaccharide, contributing to the structural integrity of the cell wall. Our data illustrate that the pharmacological and genetic interference with PECTIN METHYL ESTERASEs (PMEs) abolishes RALF1-induced root growth repression. Our data suggest that positively charged RALF1 peptides bind negatively charged, demethylated pectin with high avidity. We illustrate that the RALF1 association with demethylated pectin is required for its FERONIA-dependent perception, contributing to the control of the extracellular matrix and the regulation of plasma membrane dynamics. Notably, this mode of action is independent of the FER-dependent extracellular matrix sensing mechanism provided by FER interaction with the Leucine-Rich Repeat Extensin (LRX) proteins. We propose that the methylation status of pectin acts as a conceptualizing signalling scaffold for RALF peptides, linking extracellular matrix dynamics to peptide hormone-mediated responses.

### Competing Interest Statement

The authors have declared no competing interest.

Pectin methylesterase activity is required for RALF1 peptide signalling output

세포벽-펙틴-탈메틸화-상태가-ralf1-펩타이드-신호-전달에-필수적임


세포벽 펙틴 탈메틸화 상태가 RALF1 펩타이드 신호 전달에 필수적임
