
차 식물 뿌리에서 테아닌 합성, 수송 및 조절은 다세포 구획화 특성을 가지며, 전사 인자와 표적 유전자의 높은 공동 발현이 필요하지 않음.


coremsg

Root-synthesized secondary metabolites are critical quality-conferring compounds of foods, plant-derived medicines, and beverages. However, information at a single-cell level on root-specific secondary metabolism remains largely unexplored. L-theanine, an important quality component of tea, is primarily synthesized in roots, from which it is then transported to new tea shoots. In this study, we present a single-cell RNA sequencing (scRNA-seq)-derived map for the tea root, which enabled cell-type-specific analysis of glutamate and ethylamine (two precursors of theanine biosynthesis) metabolism, and theanine biosynthesis, storage, and transport. Our findings support a model in which the theanine biosynthesis pathway occurs via multicellular compartmentation and does not require high co-expression levels of transcription factors and their target genes within the same cell cluster. This study provides novel insights into theanine metabolism and regulation, at the single-cell level, and offers an example for studying root-specific secondary metabolism in other plant systems.

### Competing Interest Statement

The authors have declared no competing interest.

Root-specific secondary metabolism at the single-cell level: a case study of theanine metabolism and regulation in the roots of tea plants (Camellia sinensis)

차-식물-뿌리에서-단일-세포-수준의-특이적-이차-대사-차-식물-camellia-sinensis-의-테아닌-대사-및-조절에-대한-사례-연구


차 식물 뿌리에서 단일 세포 수준의 특이적 이차 대사 - 차 식물(Camellia sinensis)의 테아닌 대사 및 조절에 대한 사례 연구
