Lipids Regulate Mammalian Adenylyl Cyclase Activities via Direct Receptor Interactions
Lipids act as direct receptor ligands that modulate the activity of mammalian membrane-bound adenylyl cyclases, establishing a new level of regulation for cAMP biosynthesis.
암세포에서 젖산탈수소효소에 의한 해당과정 조절의 동역학적 및 열역학적 통찰과 삼탄소산회로 및 산화적 인산화에 미치는 영향 규명
젖산탈수소효소 억제는 해당과정, 삼탄소산회로, 산화적 인산화 모두를 억제하는 것으로, 이는 효소 동역학과 열역학적 상호작용을 통해 이루어진다.
S. aureus 펩티도글리칸 가수분해효소 lysostaphin과 LytM의 기질 특이성 재평가
lysostaphin과 LytM은 S. aureus 펩티도글리칸의 D-Ala-Gly 교차결합 부위를 선호적으로 가수분해한다.
Deciphering the Substrate Specificities of the Major Staphylococcus aureus Peptidoglycan Hydrolases Lysostaphin and LytM
The major Staphylococcus aureus peptidoglycan hydrolases lysostaphin and LytM display distinct substrate specificities, with lysostaphin preferring the D-Ala-Gly cross-link in mature peptidoglycan, while LytM can also cleave the D-alanyl-glycine bond in addition to glycyl-glycine bonds.
Allosteric Inhibition of Trypanosomatid Pyruvate Kinases by a Camelid Single-Domain Antibody
A camelid single-domain antibody (sdAb42) selectively binds and stabilizes the inactive T-state conformation of trypanosomatid pyruvate kinases, thereby inhibiting their enzymatic activity through an allosteric mechanism.
Maintaining Fluorescence of Endogenously Expressed Proteins During SDS-PAGE for Rapid and Cost-Effective Detection
In-gel fluorescence (IGF) detection is a rapid, cost-effective, and quantitative alternative to immunoblotting for visualizing endogenously expressed fluorescent proteins in cell extracts.
High-Resolution Structure of the Lysosomal Heparan-α-Glucosaminide N-Acetyltransferase (HGSNAT) Reveals Insights into Mucopolysaccharidosis IIIC
The high-resolution cryo-EM structure of the lysosomal membrane protein HGSNAT in complex with acetyl-CoA provides critical insights into the molecular basis of its N-acetyltransferase activity and the impact of mutations that cause the rare lysosomal storage disorder mucopolysaccharidosis IIIC.
微小管のポリグリシル化を触媒するTTLL10酵素は、チューブリンのグルタミル化によって活性化され、ポリグリシル化によって阻害される
TTLL10酵素は、既存のモノグリシル化チューブリンに対してのみ高い親和性を示し、ポリグリシル化鎖を伸長する。一方で、ポリグリシル化は逆にTTLL10の微小管への結合を阻害する。また、先行するグルタミル化はTTLL10の微小管結合を促進する。これにより、微小管上の修飾パターンが制御される。
DTX3L Ubiquitin Ligase Catalyzes Direct Ubiquitination of Single-Stranded Nucleic Acids
DTX3L, a member of the Deltex ubiquitin ligase family, can directly ubiquitinate the 3' hydroxyl group of single-stranded DNA and RNA, expanding the repertoire of non-proteinaceous substrates that undergo ubiquitination.
단일 이가 금속 이온 의존성 및 히스티딘 촉진 His-Me 가족 I-PpoI 핵산분해효소 촉매 과정의 결정 내 관찰
His-Me 핵산분해효소는 단일 이가 금속 이온과 보존된 히스티딘 잔기를 이용하여 DNA 가수분해를 촉매한다.
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