
完全な因子設計により、微生物相互作用の複雑性を解明し、最適な微生物コンソーシアムを見出すことができる。


coremsg

Constructing combinatorially complete species assemblages is often necessary to dissect the complexity of microbial interactions and to find optimal microbial consortia. At the moment, this is accomplished through either painstaking, labor intensive liquid handling procedures, or through the use of state-of-the-art microfluidic devices. Here we present a simple, rapid, low-cost, and highly accessible liquid handling methodology for assembling all possible combinations of a library of microbial strains, which can be implemented with basic laboratory equipment. To demonstrate the usefulness of this methodology, we construct a combinatorially complete set of consortia from a library of eight Pseudomonas aeruginosa strains, and empirically measure the community-function landscape of biomass productivity, identify the highest yield community, and dissect the interactions that lead to its optimal function. This easy to implement, inexpensive methodology will make the assembly of combinatorially complete microbial consortia easily accessible for all laboratories.

### Competing Interest Statement

The authors have declared no competing interest.

Full factorial construction of synthetic microbial communities

完全な因子設計による合成微生物コミュニティの構築

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2つの細菌B12オーキシトロフが、異なるB12構成要素を回収し合い、共同してB12を合成することで、B12欠乏を解決する。


Two species of auxotrophic marine bacteria are shown to share precursors to synthesize the essential cofactor vitamin B12, and such ligand cross-feeding may be a common phenomenon in the ocean and other ecosystems.

Ligand cross-feeding resolves bacterial vitamin B12 auxotrophies - Nature

細菌のビタミンb12オーキシトロフィーを解決するリガンドクロスフィーディング


細菌のビタミンB12オーキシトロフィーを解決するリガンドクロスフィーディング
