
벨리마운트-펄스 추적 기법을 통해 최대 16시간 동안 살아있는 암컷 복부 내에서 난소 발생의 핵심 사건들을 연속적이고 비침습적으로 관찰할 수 있다.


coremsg

Quantitative live imaging is a valuable tool that offers insights into cellular dynamics. However, many fundamental biological processes are incompatible with current live imaging modalities. Drosophila oogenesis is a well-studied system that has provided molecular insights into a range of cellular and developmental processes. The length of the oogenesis coupled with the requirement for inputs from multiple tissues has made long-term culture challenging. Here, we have developed Bellymount-Pulsed Tracking (Bellymount-PT), which allows continuous, non-invasive live imaging of Drosophila oogenesis inside the female abdomen for up to 16 hours. Bellymount-PT improves upon the existing Bellymount technique by adding pulsed anesthesia with periods of feeding that support the long-term survival of flies during imaging. Using Bellymount-PT we measure key events of oogenesis including egg chamber growth, yolk uptake, and transfer of specific proteins to the oocyte during nurse cell dumping with high spatiotemporal precision within the abdomen of a live female.

SUMMARY STATEMENT Bellymount-Pulsed Tracking is a novel tool to capture in vivo dynamics for longer duration in Drosophila oogenesis.

### Competing Interest Statement

The authors have declared no competing interest.

Bellymount-Pulsed Tracking: A Novel Approach for Real-Time In vivo Imaging of Drosophila Abdominal Tissues

실시간-체내-드로소필라-난소-발생-관찰을-위한-새로운-접근법-벨리마운트-펄스-추적


실시간 체내 드로소필라 난소 발생 관찰을 위한 새로운 접근법: 벨리마운트-펄스 추적
