
세틴은 엑소시스트 복합체의 세포막 표적화를 직접적으로 조절하여 세포 분열 과정에서 외분비를 조절한다.


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Septins can function as scaffolds for protein recruitment, membrane-bound diffusion barriers, or membrane curvature sensors. Septins are important for cytokinesis, but their exact roles are still obscure. In fission yeast, four septins (Spn1 to Spn4) accumulate at the rim of the division plane as rings. The octameric exocyst complex, which tethers exocytic vesicles to the plasma membrane, exhibits a similar localization and is essential for plasma membrane deposition during cytokinesis. Without septins, the exocyst spreads across the division plane but absent from the rim during septum formation. These results suggest that septins and the exocyst physically interact for proper localization. Indeed, we predicted six pairs of direct interactions between septin and exocyst subunits by AlphaFold2 ColabFold, most of them are confirmed by co-immunoprecipitation and yeast two-hybrid assays. Exocyst mislocalization results in mistargeting of secretory vesicles and their cargos, which leads to cell-separation delay in septin mutants. Our results indicate that septins guide the targeting of exocyst complex on the plasma membrane for vesicle tethering during cytokinesis through direct physical interactions.

### Competing Interest Statement

The authors have declared no competing interest.

*   Co-IP
    :   Co-immunoprecipitation
    EMCCD
    :   Electron-multiplying charge-coupled device
    FPS
    :   Frame per second
    FRAP
    :   Fluorescence recovery after photobleaching
    NA
    :   Numerical aperture
    PB
    :   Phloxin B
    pLDDT
    :   Predicted Local-Distance Difference Test
    ROI
    :   Region of interest
    SPB
    :   Spindle pole body
    TIRF
    :   Total internal reflection fluorescence
    TRAPP-II
    :   Transport particle protein-II
    WT
    :   Wild type

Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis

세포-분열-과정에서-세틴과-엑소시스트-복합체-간의-물리적-상호작용을-통한-외분비-기능


세포 분열 과정에서 세틴과 엑소시스트 복합체 간의 물리적 상호작용을 통한 외분비 기능
