cybersecurity-threat-analysis-and-attack-simulations-for-unmanned-aerial-vehicle-networks

無人航空機ネットワークのサイバーセキュリティ脅威分析と攻撃シミュレーション

무인-항공기-네트워크에-대한-사이버-보안-위협-분석-및-공격-시뮬레이션

secure-data-distribution-and-reconstruction-using-rainbow-antimagic-graph-coloring-in-secret-share-schemes

秘密共有スキームにおけるレインボーアンチマジック-グラフ着色を用いたデータセキュリティの強化

비밀-공유-배포-및-복원을-위한-레인보우-반자기-그래프-색칠을-통한-데이터-보안-강화

satellite-spectroscopy-and-machine-learning-for-large-scale-sugarcane-health-monitoring

サトウキビの衛星分光法と機械学習を用いた健康モニタリング-レビュー


アミノ酸輸送体は、単一の適応変異によって基質範囲を大幅に拡大できることが示された。これにより、生物は新しい生態的地位を探索できるようになる。


coremsg

The emergence of new protein functions is crucial for the evolution of organisms. This process has been extensively researched for soluble enzymes, but it is largely unexplored for membrane transporters, even though the ability to acquire new nutrients from a changing environment requires evolvability of transport functions. Here, we demonstrate the importance of environmental pressure in obtaining a new activity or altering a promiscuous activity in members of the Amino acid-Polyamine-organoCation (APC)-type yeast amino acid transporters family. We identify APC members that have broader substrate spectra than previously described. Using in vivo experimental evolution, we evolve two of these transporter genes, AGP1 and PUT4 , towards new substrate specificities. Single mutations on these transporters are found to be sufficient for expanding the substrate range of the proteins, while retaining the capacity to transport all original substrates. Nonetheless, each adaptive mutation comes with a distinct effect on the fitness for each of the original substrates, illustrating a trade-off between the ancestral and evolved functions. Collectively, our findings reveal how substrate-adaptive mutations in membrane transporters contribute to fitness and provide insights into how organisms can use transporter evolution to explore new ecological niches.

### Competing Interest Statement

The authors have declared no competing interest.

Fitness landscape of substrate-adaptive mutations in evolved APC transporters

アミノ酸適応変異のフィットネスランドスケープ-進化したapc輸送体の解析


アミノ酸適応変異のフィットネスランドスケープ:進化したAPC輸送体の解析


title_rewrite

アミノ酸輸送体の基質適応進化