
DC-iDEP技術を用いることで、グルコース刺激の有無によって変化するインスリン分泌小胞のサブポピュレーションを高分解能で分離・解析できる。


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Organelle heterogeneity and inter-organelle associations within a single cell contribute to the limited sensitivity of current organelle separation techniques, thus hindering organelle subpopulation characterization. Here we use direct current insulator-based dielectrophoresis (DC-iDEP) as an unbiased separation method and demonstrate its capability by identifying distinct distribution patterns of insulin vesicles from pancreatic β-cells. A multiple voltage DC-iDEP strategy with increased range and sensitivity has been applied, and a differentiation factor (ratio of electrokinetic to dielectrophoretic mobility) has been used to characterize features of insulin vesicle distribution patterns. We observed a significant difference in the distribution pattern of insulin vesicles isolated from glucose-stimulated cells relative to unstimulated cells, in accordance with functional maturation of vesicles upon glucose stimulation, and interpret this to be indicative of high-resolution separation of vesicle subpopulation. DC-iDEP provides a path for future characterization of subtle biochemical differences of organelle subpopulations within any biological system.

### Competing Interest Statement

MAH declares a conflict of interest with CBio where he serves as the CSO and with Hayes Diagnostics, Inc. where he serves as COB, CEO & CSO. All other authors have no conflict of interest to declare.

iDEP-assisted isolation of insulin secretory vesicles

インスリン分泌小胞の高分解能分離を可能にするidep技術の活用


インスリン分泌小胞の高分解能分離を可能にするiDEP技術の活用
