
複数のインフルエンザ亜型に対する交差反応性の体液性免疫応答を誘導することを目的とした次世代インフルエンザワクチンの開発


coremsg

Next-generation influenza vaccines aim to elicit cross-reactive humoral responses to multiple influenza subtypes. Such increased breadth would not only improve seasonal vaccines but may afford ‘universal’ protection against influenza subtypes including those with pandemic potential. Here, we describe a “beads-on-a-string” (BOAS) immunogen, that tandemly links up to eight distinct hemagglutinin (HA) head domains from circulating and non-circulating influenzas. These BOAS are immunogenic in the murine model and elicit comparable serum responses to each individual component. Notably, we also find that BOAS elicit cross-reactive, and neutralizing responses to influenza subtypes not included in the immunizing immunogen. Furthermore, BOAS conjugation to protein-based ferritin nanoparticles does not significantly augment serum responses suggesting that our BOAS platform is sufficient for eliciting cross-reactive responses without off-target effects induced by the nanoparticle scaffold. This mix-and-match immunogen design strategy is a robust platform for eliciting responses to multiple influenza subtypes via a single immunogen, and a potential platform for other viral glycoproteins.

### Competing Interest Statement

DTL and AGS have filed a provisional patent covering the work described here.

A modular platform to display multiple hemagglutinin subtypes on a single immunogen

複数のヘマグルチニン亜型を単一の免疫原に表示するためのモジュール式プラットフォーム