
ショウジョウバエ幼虫の逃避行動において、下行性GABAニューロンSeIN128が、上流のBasinニューロンとA00cニューロンからの入力を受け、ローリング行動の終了と這い行動への移行を促進する抑制的フィードバックを形成している。


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Escape behaviors help animals avoid harm from predators and other threats in the environment. Successful escape relies on integrating information from multiple stimulus modalities (of external or internal origin) to compute trajectories toward safe locations, choose between actions that satisfy competing motivations, and execute other strategies that ensure survival. To this end, escape behaviors must be adaptive. When a Drosophila melanogaster larva encounters a noxious stimulus, such as the focal pressure a parasitic wasp applies to the larval cuticle via its ovipositor, it initiates a characteristic escape response. The escape sequence consists of an initial abrupt bending, a corkscrew-like rolling, and finally rapid crawling. Previous work has shown that the detection of noxious stimuli primarily relies on class IV multi dendritic arborization neurons (Class IV neurons) located beneath the body wall, and more recent studies have identified several important components in the nociceptive neural circuitry involved in rolling. However, the neural mechanisms that underlie the rolling-escape sequence remain unclear. Here we present both functional and anatomical evidence suggesting that bilateral descending neurons within the subesophageal zone of D. melanogaster larva play a crucial role in regulating the termination of rolling and subsequent transition to escape crawling. We demonstrate that these descending neurons (designated SeIN128) are inhibitory and receive inputs from a second-order interneuron upstream (Basin-2) and an ascending neuron downstream of Basin-2 (A00c). Together with optogenetic experiments showing that co-activation of SeIN128 neurons and Basin-2 influence the temporal dynamics of rolling, our findings collectively suggest that the ensemble of SeIN128, Basin-2, and A00c neurons forms a GABAergic feedback loop onto Basin-2, which inhibits rolling and thereby facilitates the shift to escape crawling.

### Competing Interest Statement

The authors have declared no competing interest.

The original contributions presented in this study are included in the article/supplemental material; further inquiries may be directed to the corresponding author.

Feedback inhibition by a descending GABAergic neuron regulates timing of escape behavior in Drosophila larvae

寄生蜂の産卵行動に対する防御反応のタイミングを調整する下行性gabaニューロンによる抑制フィードバック


寄生蜂の産卵行動に対する防御反応のタイミングを調整する下行性GABAニューロンによる抑制フィードバック
