translation-techniques-prediction-study-for-machine-translation-optimization

ninerec-a-benchmark-dataset-suite-for-evaluating-transferable-recommendation

generating-tables-from-text-by-conditional-question-answering

radar-lidar-localization-improvement-using-learned-icp-weights

multi-level-explanations-for-generative-language-models-a-framework-for-improved-understanding

深層非負値行列因子分解とベータダイバージェンス

towards-single-system-illusion-in-software-defined-vehicles-automated-ai-powered-workflow

dynamon-motion-aware-fast-and-robust-camera-localization-for-dynamic-neural-radiance-fields


U2OS細胞内のMyosin 10は、細胞全体の5.4%しかフィロポディアに局在しているが、1本のフィロポディア内には数百分子も存在する。一部のフィロポディア先端では、アクチンフィラメントの結合サイトを超えるMyosin 10が蓄積しており、フィロポディア内でMyosin 10の詰まりが起こる可能性がある。


coremsg

Myosin 10 (Myo10) is a motor protein well known for its role in filopodia formation. Although Myo10-driven filopodial dynamics have been characterized, there is no information about the absolute number of Myo10 molecules during the filopodial lifecycle. To better understand molecular stoichiometries and packing restraints in filopodia, we measured Myo10 abundance in these structures. Here we combined SDS-PAGE densitometry with epifluorescence microscopy to quantitate HaloTag-labeled Myo10 in U2OS cells. About 6% of total intracellular Myo10 localizes to filopodia, where it is enriched at opposite ends of the cell. Hundreds of Myo10 are found in a typical filopodium, and their distribution across filopodia is log-normal. Some filopodial tips even contain more Myo10 than accessible binding sites on the actin filament bundle. Live-cell movies reveal a dense cluster of over a hundred Myo10 molecules that initiates filopodial elongation. Hundreds of Myo10 molecules continue to accumulate during filopodial growth, but that accumulation ceases when filopodia begin to retract. Rates of filopodial elongation, second-phase elongation, and retraction are inversely related to Myo10 quantities. Our estimates of Myo10 molecules in filopodia provide insight into the physics of packing Myo10, its cargo, and other filopodia-associated proteins in narrow membrane compartments. Our protocol provides a framework for future work analyzing Myo10 abundance and distribution upon perturbation.

### Competing Interest Statement

RSR is a consultant for Cyntegron Therapeutics.

Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actin

u2osの細胞内でmyosin-10が密集し-アクチンフィラメントの束に詰まりを引き起こす可能性がある


U2OSの細胞内でMyosin 10が密集し、アクチンフィラメントの束に詰まりを引き起こす可能性がある


title_rewrite

細胞内のMyosin 10の量と分布