
드로소필라 MSL1 단백질의 N-말단 영역은 roX RNA와의 상호작용과 X 염색체에 선량 보상 복합체 모집에 중요한 역할을 한다.


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The male-specific dosage compensation complex (DCC), which consists of five proteins and two non-coding roX RNAs, is necessary for the transcriptional enhancement of X-linked genes to compensate for the sex chromosome monosomy in Drosophila XY males compared with XX females. The MSL1 and MSL2 proteins form the heterotetrameric core of DCC and are critical for the specific recruitment of the DCC to the high-affinity “entry” sites (HAS) on the X chromosome. In this study, we demonstrated that the N-terminal region of MSL1 is critical for stability and functions of MSL1. Amino acid deletions and substitutions in the N-terminal region of MSL1 strongly affect both the interaction with roX2 RNA and DCC binding to HAS on the X chromosome. In particular, substitution of the conserved N-terminal amino-acids 3-7 in MSL1 (MSL1GS) affects dosage compensation similar to the inactivation of genes encoding roX RNAs. In addition, MSL1GS binds to promoters such as MSL1WT but does not co-bind with MSL2 and MSL3 to X chromosomal HAS. However, overexpression of MSL2 partially restores the functional activity of MSL1GS in dosage compensation. Thus, the interaction of MSL1 with roX RNA is critical for the efficient assembly of DCCs on HAS of the male X chromosome.

### Competing Interest Statement

The authors have declared no competing interest.

N-Terminus of Drosophila Melanogaster MSL1 Is Critical for Dosage Compensation

드로소필라-msl1의-n-말단이-dna-결합-단백질과-상호작용하며-x-염색체에-선량-보상-복합체-모집에-중요한-역할을-한다


드로소필라 MSL1의 N-말단이 DNA 결합 단백질과 상호작용하며 X 염색체에 선량 보상 복합체 모집에 중요한 역할을 한다
