
ntsm은 정렬 없이 k-mer 카운팅을 통해 다양한 염기서열 데이터 유형에서 효율적으로 샘플 스왑을 탐지할 수 있는 도구이다.


coremsg

Background Due to human error, sample swapping in large cohort studies with heterogeneous data types (e.g. mix of Oxford Nanopore, Pacific Bioscience, Illumina data, etc .) remains a common issue plaguing large-scale studies. At present, all sample swapping detection methods require costly and unnecessary (e.g. if data is only used for genome assembly) alignment, positional sorting, and indexing of the data in order to compare similarly. As studies include more samples and new sequencing data types, robust quality control tools will become increasingly important.

Findings The similarity between samples can be determined using indexed k -mer sequence variants. To increase statistical power, we use coverage information on variant sites, calculating similarity using a likelihood ratio-based test. Per sample error rate, and coverage bias ( i . e . missing sites) can also be estimated with this information, which can be used to determine if a spatially indexed PCA-based pre-screening method can be used, which can greatly speed up analysis by preventing exhaustive all-to-all comparisons.

Conclusions Because this tool processes raw data, is faster than alignment, and can be used on very low coverage data, it can save an immense degree of computational resources in standard QC pipelines. It is robust enough to be used on different sequencing data types, important in studies that leverage the strengths of different sequencing technologies. In addition to its primary use case of sample-swap detection, this method provides other useful information useful in QC, such as error rate and coverage bias, as well as population-level PCA ancestry analysis visualization.

### Competing Interest Statement

The authors have declared no competing interest.

ntsm: an alignment-free, ultra low coverage, sequencing technology agnostic, intraspecies sample comparison tool for sample swap detection

다양한-염기서열-데이터-유형에-적용-가능한-정렬-없는-초저-커버리지-샘플-비교-도구-ntsm-샘플-스왑-탐지를-위한-기술-독립적-솔루션


다양한 염기서열 데이터 유형에 적용 가능한 정렬 없는 초저 커버리지 샘플 비교 도구 ntsm: 샘플 스왑 탐지를 위한 기술 독립적 솔루션
