
신속하고 효율적인 항체 스크리닝 시스템을 개발하여 치료 및 진단용 항체를 신속하게 발견할 수 있다.


coremsg

Antibodies are powerful tools for the therapy and diagnosis of various diseases. In addition to conventional hybridoma-based screening, recombinant antibody-based screening has become a common choice; however, its application is hampered by two factors: 1) screening starts only after Ig gene cloning and recombinant antibody production, and 2) the antibody is composed of paired chains, heavy and light, commonly expressed from two independent expression vectors. Here, we introduce a method for the rapid screening of recombinant monoclonal antibodies by establishing a Golden Gate-based dual-expression vector and in vivo expression of membrane-bound antibodies. Using this system, we demonstrated the efficient isolation of influenza cross-reactive antibodies with high affinity from mouse germinal center B cells over 4 days. This system is particularly useful for isolating therapeutic or diagnostic antibodies (e.g., during foreseen pandemics).

Impact Statement A Golden Gate-based dual-expression vector enables rapid screening, facilitating efficient isolation of high-affinity cross-reactive antibodies for therapeutic or diagnostic use, offering a crucial advance for pandemic preparedness.

### Competing Interest Statement

The authors have declared no competing interest.

Development of a new genotype–phenotype linked antibody screening system

신속한-유전형-표현형-연계-항체-스크리닝-시스템-개발


신속한 유전형-표현형 연계 항체 스크리닝 시스템 개발
