
아메바양 세포는 안정적인 점착 접합체 없이도 기질 강성 차이를 감지하고 이에 따라 방향성 이동을 할 수 있다.


coremsg

Cell migration towards stiff substrates has been coined as durotaxis and implicated in development, wound healing and cancer, where complex interplays between immune and non-immune cells are present. Compared to the emerging mechanisms underlying the strongly adhesive mesenchymal durotaxis, little is known about whether immune cells - migrating in amoeboid mode - could follow mechanical cues. Here we develop an imaging-based confined migration device with stiffness gradient. By tracking live cell trajectory and analyzing the directionality of T cells and neutrophils, we observe that amoeboid cells can durotax. We further delineate the underlying mechanism to involve non-muscle myosin IIA (NMIIA) polarization towards the soft-matrix-side but may not require differential actin flow up-or down-stiffness gradient. Using the protista Dictyostelium , we demonstrate the evolutionary conservation of amoeboid durotaxis. Finally, these experimental phenomena are theoretically captured by an active gel model capable of mechanosensing. Collectively, these results may shed new lights on immune surveillance and recently identified confined migration of cancer cells, within the mechanically inhomogeneous tumor microenvironment or the inflamed fibrotic tissues.

### Competing Interest Statement

The authors have declared no competing interest.

Amoeboid cells undergo durotaxis with soft end polarized NMIIA

아메바양-세포가-연성-기질-극성화-nmiia를-통해-듀로택시를-거친다


아메바양 세포가 연성 기질 극성화 NMIIA를 통해 듀로택시를 거친다
