
DRAM 시스템은 탈아미노화 효소와 5-메틸시토신 판독 단백질을 결합하여 전사체 전반에 걸쳐 5-메틸시토신 위치를 효과적이고 포괄적으로 확인할 수 있는 새로운 방법을 제공한다.


coremsg

5-Methylcytosine (m5C) is one of the major post-transcriptional modifications in mRNA and is highly involved in the pathogenesis of various diseases. However, the capacity of existing assays for accurately and comprehensively transcriptome-wide m5C mapping still needs improvement. Here, we develop a detection method named DRAM (deaminase and reader protein assisted RNA methylation analysis), in which deaminases (APOBEC1 and TadA-8e) are fused with m5C reader proteins (ALYREF and YBX1) to identify the m5C sites through deamination events neighboring the methylation sites. This antibody-free and bisulfite-free approach provides transcriptome-wide editing regions which are highly overlapped with the publicly available BS-seq datasets and allows for a more stable and comprehensive identification of the m5C loci. In addition, DRAM system even supports ultra-low input RNA (10ng) and monitor the dynamic accumulation of cellular m5C. We anticipate that the DRAM system could pave the way for uncovering further biological functions of m5C modifications.

### Competing Interest Statement

The authors have declared no competing interest.

Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing

항체-및-바이설파이트-처리-없이-전사체-전반에-걸쳐-5-메틸시토신을-효과적으로-검출하는-새로운-방법-개발


항체 및 바이설파이트 처리 없이 전사체 전반에 걸쳐 5-메틸시토신을 효과적으로 검출하는 새로운 방법 개발
