
Pseudomonas sp. 균주 JS3066은 1-나프틸아민을 유일한 탄소 및 질소원으로 이용하여 분해할 수 있으며, 이 과정에서 1-나프틸아민을 글루타밀화하는 효소 NpaA1의 구조와 기능을 규명하였다.


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1-Naphthylamine (1NA), which is harmful to human and aquatic animals, has been used widely in the manufacturing of dyes, pesticides, and rubber antioxidants. Nevertheless, little is known about its environmental behavior and no bacteria have been reported to use it as the growth substrate. Herein, we describe a pathway for 1NA degradation in isolate Pseudomonas sp. strain JS3066, determine the structure and mechanism of the enzyme NpaA1 that catalyzes the initial reaction, and reveal how the pathway evolved. From genetic and enzymatic analysis, a cluster of 5 genes encoding a dioxygenase system was determined to be responsible for the initial steps in 1NA degradation through glutamylation of 1NA. The γ-glutamylated 1NA was subsequently oxidized to 1,2-dihydroxynaphthalene which was further degraded by the well-established pathway of naphthalene degradation via catechol. Enzymatic analysis showed that NpaA1 catalyzed conversion of various anilines and naphthylamine derivatives. Structural and biochemical studies of NpaA1 revealed that the broad substrate specificity of NpaA1 is due to a large hydrophobic pocket, which is different from type I glutamine synthetase (GSI). The findings enhance understanding of degrading polycyclic aromatic amines, and will also enable the application of bioremediation at naphthylamine contaminated sites.

### Competing Interest Statement

The authors have declared no competing interest.

Discovery of the 1-naphthylamine biodegradation pathway reveals an enzyme that catalyzes 1-naphthylamine glutamylation

1-나프틸아민-생분해-경로-발견을-통한-1-나프틸아민-글루타밀화-효소의-구조와-기능-규명


1-나프틸아민 생분해 경로 발견을 통한 1-나프틸아민 글루타밀화 효소의 구조와 기능 규명
