
The rete ovarii is a complex, multi-regional epithelial structure directly connected to the ovary that plays an important role in ovary development and homeostasis through secretion of proteins into the ovary.


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The rete ovarii (RO) is an appendage of the ovary that has been given little attention. Although the RO appears in drawings of the ovary in early versions of Gray’s Anatomy, it disappeared from recent textbooks, and is often dismissed as a functionless vestige in the adult ovary. Using PAX8 immunostaining and confocal microscopy, we characterized the fetal development of the RO in the context of the ovary. The RO consists of three distinct regions that persist in adult life, the intraovarian rete (IOR), the extraovarian rete (EOR), and the connecting rete (CR). While the cells of the IOR appear to form solid cords within the ovary, the EOR rapidly develops into a convoluted tubular epithelium ending in a distal dilated tip. Cells of the EOR are ciliated and exhibit cellular trafficking capabilities. The CR, connecting the EOR to the IOR, gradually acquires tubular epithelial characteristics by birth. Using microinjections into the distal dilated tip of the EOR, we found that luminal contents flow towards the ovary. Mass spectrometry revealed that the EOR lumen contains secreted proteins potentially important for ovarian function. We show that the cells of the EOR are closely associated with vasculature and macrophages, and are contacted by neuronal projections, consistent with a role as a sensory appendage of the ovary. The direct proximity of the RO to the ovary and its integration with the extraovarian landscape suggest that it plays an important role in ovary development and homeostasis.

### Competing Interest Statement

The authors have declared no competing interest.

Rediscovering the Rete Ovarii: a secreting auxiliary structure to the ovary

characterization-of-the-rete-ovarii-a-secretory-appendage-of-the-ovary-with-potential-roles-in-ovarian-function


Characterization of the Rete Ovarii: A Secretory Appendage of the Ovary with Potential Roles in Ovarian Function
