
Sequencing and analysis of the complete genome of the Amazonian fruit tree Theobroma grandiflorum (cupuassu) provides insights into its genomic evolution, unique traits, and genetic relationship with the closely related cacao (Theobroma cacao).


coremsg

Background Theobroma grandiflorum (Malvaceae), known as cupuassu, is a tree indigenous to the Amazon Basin, valued for its large fruits and seed-pulp, contributing notably to the Amazonian bioeconomy. The seed-pulp is utilized in desserts and beverages, and its seed butter is used in cosmetics. Here, we present the sequenced telomere-to-telomere cupuassu genome, disclosing features of the genomic structure, evolution, and phylogenetic relationships within the Malvaceae.

Results The cupuassu genome spans 423 Mb, encodes 31,381 genes distributed in the ten chromosomes, and it exhibits approximately 65% gene synteny with the T. cacao genome, reflecting a conserved evolutionary history, albeit punctuated with unique genomic variations. The main changes are pronounced by bursts of long-terminal repeats retrotransposons expansion at post-species divergence, retrocopied and singleton genes, and gene families displaying distinctive patterns of expansion and contraction. Furthermore, positively selected genes are evident, particularly among retained and dispersed, tandem and proximal duplicated genes associated to general fruit and seed traits and defense mechanisms, supporting the hypothesis of potential episodes of subfunctionalization and neofunctionalization following duplication, and impact from distinct domestication process. These genomic variations may underpin the differences observed in fruit and seed morphology, ripening, and disease resistance between cupuassu and the other Malvaceae species.

Conclusions Sequencing the cupuassu genome offers a foundational resource for both breeding and conservation efforts, yielding insights into the evolution and diversity within the genus Theobroma .

Core ideas 

AUTHOR SUMMARY Cupuassu, a fruit-bearing tree from the Amazon, is prized for its nutritious fruits and seeds, widely used in food and cosmetics. In this study, we sequenced the complete genome of cupuassu to understand its development, unique traits, and genetic relationship with cacao and other related species. The cupuassu genome shares a high similarity with cacao, but it also exhibits distinctive features. Notably, repetitive DNA elements have significantly influenced its genomic structure. Furthermore, specific genes responsible for its fruit and seed characteristics, as well as disease resistance, were identified. Overall, this research not only deepens our knowledge of cupuassu genetics but also illuminates broader aspects of plant evolution and diversity in the Amazon. It lays the groundwork for advanced breeding programs and promises to contribute significantly to the Amazonian bioeconomy. Ultimately, these findings have important implications for agriculture and conservation, highlighting the intricate processes of plant adaptation and evolution.

### Competing Interest Statement

The authors have declared no competing interest.

*   BUSCO
    :   Benchmarking Universal Single-Copy Orthologs
    CDS
    :   coding sequence
    CTAB
    :   Cetyltrimethylammonium Bromide
    FP
    :   Frosty pod
    GO
    :   Gene Ontology
    HiC
    :   Chromosome conformation capture techniques
    HMW
    :   High molecular weight
    Ka
    :   non-synonymous nucleotide substitutions
    Ks
    :   synonymous substitutions
    LAI
    :   LTR Assembly Index
    LARD
    :   Large Retrotransposon Derivatives
    lncRNAs
    :   long non-coding RNAs
    LTR-RT
    :   Long Terminal Repeat Retrotransposons
    miRNAs
    :   microRNAs
    mya
    :   million years ago
    QTL
    :   Quantitative trait locus
    rDNA
    :   ribosomal DNA
    snoRNAs
    :   small nucleolar RNAs
    snRNAs
    :   small nuclear RNAs
    sRNAs
    :   small RNAs
    T2T
    :   telomere-to-telomere
    TE
    :   Transposable Elements
    TRIM
    :   Terminal-repeat Retrotransposons in Miniature
    tRNAs
    :   transfer RNAs
    WBD
    :   Witches’ broom disease
    WGD
    :   Whole-genome duplication

Genomic decoding of Theobroma grandiflorum (cupuassu) at chromosomal scale: Evolutionary insights for horticultural innovation

chromosome-scale-genome-assembly-of-the-amazonian-fruit-tree-theobroma-grandiflorum-reveals-insights-into-genomic-evolution-and-agronomic-traits


Chromosome-Scale Genome Assembly of the Amazonian Fruit Tree Theobroma grandiflorum Reveals Insights into Genomic Evolution and Agronomic Traits
