
The author explores the multistep process of stable microtubule bundle formation in quiescent yeast, highlighting the sequential actions of specific kinesins and kinetochore interactions. The main thesis is to provide a detailed molecular-scale description of the life cycle of a stable microtubule structure in vivo.


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Cells fine-tune microtubule assembly in both space and time, to give rise to distinct edifices with specific cellular functions. In proliferating cells, microtubules are highly dynamics, and proliferation cessation often leads to their stabilization. One of the most stable microtubule structures identified to date is the nuclear bundle assembled in quiescent yeast. In this report, we characterize the original multistep process driving the assembly of this structure. This Aurora B-dependent mechanism follows a precise temporality that relies on the sequential actions of kinesin-14, kinesins-5 and involves both microtubule-kinetochore and kinetochore-kinetochore interactions. Upon quiescence exit, the microtubule bundle is disassembled via a cooperative process involving kinesin-8 and its full disassembly is required prior to cells re-entry into proliferation. Overall, our study provides the first description, at the molecular scale, of the entire life cycle of a stable microtubule structure in vivo , and sheds light on its physiological function.

### Competing Interest Statement

The authors have declared no competing interest.

*   MT
    :   microtubule
    SPB
    :   spindle pole body
    GTP
    :   guanosine triphosphate
    MTOC
    :   microtubule organizing center
    ɣ-TuC
    :   ɣ-tubulin complex
    MAPs
    :   microtubule associated proteins
    Q-nMT bundle
    :   quiescence specific nuclear microtubule bundle
    CPC
    :   chromosome passenger complex

A stable microtubule bundle formed through an orchestrated multistep process controls quiescence exit

Unveiling-the-Process-of-Stable-Microtubule-Bundle-Formation-in-Quiescent-Yeast


Unveiling the Process of Stable Microtubule Bundle Formation in Quiescent Yeast
