
BMP signaling is required to maintain the cell fate of auricular chondrocytes by preventing them from undergoing osteogenic differentiation, and disruption of this signaling leads to microtia development through activation of the PKA pathway.


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Elastic cartilage constitutes a major component of the external ear, which functions to guide sound to the middle and inner ears. Defects in auricle development cause congenital microtia, which affects hearing and appearance in patients. Mutations in several genes have been implicated in microtia development, yet, the pathogenesis of this disorder remains incompletely understood. Here, we show that Prrx1 genetically marks auricular chondrocytes in adult mice. Interestingly, BMP-Smad1/5/9 signaling in chondrocytes is increasingly activated from the proximal to distal segments of the ear, which is associated with a decrease in chondrocyte regenerative activity. Ablation of Bmpr1a in auricular chondrocytes led to chondrocyte atrophy and microtia development at the distal part. Transcriptome analysis revealed that Bmpr1a deficiency caused a switch from the chondrogenic program to the osteogenic program, accompanied by enhanced protein kinase A activation, likely through increased expression of Adcy5/8 . Inhibition of PKA blocked chondrocyte-to-osteoblast transformation and microtia development. Moreover, analysis of single-cell RNA-seq of human microtia samples uncovered enriched gene expression in the PKA pathway and chondrocyte-to-osteoblast transformation process. These findings suggest that auricle cartilage is actively maintained by BMP signaling, which maintains chondrocyte identity by suppressing osteogenic differentiation.

### Competing Interest Statement

The authors have declared no competing interest.

BMP signaling maintains auricular chondrocyte identity and prevents microtia development by inhibiting protein kinase A

bmp-signaling-maintains-auricular-chondrocyte-identity-and-prevents-microtia-development-by-inhibiting-protein-kinase-a


BMP Signaling Maintains Auricular Chondrocyte Identity and Prevents Microtia Development by Inhibiting Protein Kinase A
