
Nitric oxide selectively modulates the temporal response properties of a distinct subset of contrast-suppressed retinal ganglion cell types, reducing their response suppression and increasing their activity, without affecting their spatial receptive field properties.


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Neuromodulators have major influences on the regulation of neural circuit activity across the nervous system. Nitric oxide (NO) has been shown to be a prominent neuromodulator in many circuits and has been extensively studied in the retina. Here, it has been associated with the regulation of light adaptation, gain control, and gap junctional coupling, but its effect on the retinal output, specifically on the different types of retinal ganglion cells (RGCs), is still poorly understood. In this study, we used two-photon Ca2+ imaging and multi-electrode array (MEA) recordings to measure light-evoked activity of RGCs in the ganglion cell layer in the ex vivo mouse retina. This approach allowed us to investigate the neuromodulatory effects of NO on a cell type-level. Our findings reveal that NO selectively modulates the suppression of temporal responses in a distinct subset of contrast-suppressed RGC types, increasing their activity without altering the spatial properties of their receptive fields. Given that NO release is triggered by quick changes in light levels under photopic conditions, we propose that these RGC types signal fast contrast changes to higher visual regions. Remarkably, we found that about one-third of the RGC types, recorded using two-photon Ca2+ imaging, exhibited consistent, cell type-specific adaptational response changes throughout an experiment, independent of NO. By employing a paired-recording paradigm, we could disentangle those additional adaptational response changes from drug-induced modulations. Taken together, our research highlights the selective neuromodulatory effects of NO on RGCs and emphasizes the need of considering non-pharmacological activity changes, like adaptation, in such study designs.

### Competing Interest Statement

The authors have declared no competing interest.

Nitric oxide modulates contrast suppression in a subset of mouse retinal ganglion cells

selective-modulation-of-retinal-ganglion-cell-responses-by-nitric-oxide


Selective Modulation of Retinal Ganglion Cell Responses by Nitric Oxide
