
p97 단백질 분해 효소를 이용한 PROTAC 기술은 기존 유비퀴틴 기반 PROTAC 기술과 달리 유비퀴틴 독립적으로 특정 단백질을 분해할 수 있다.


coremsg

The p97 protein is a member of the AAA + family of ATPases. It is a mechanoenzyme that uses energy from ATP hydrolysis to promote protein unfolding and segregation actively. The unfolded products of p97 are presented to the 26S Proteasome for degradation. p97 substrate recognition is mediated by adaptors, which interact with substrates directly or indirectly through ubiquitin modifications, resulting in substrate funnelling into the central pore of the p97 hexamer and unfolding. We have engineered synthetic adaptors to target specific substrates to p97, using the extraordinary intracellular binding capabilities of camelid nanobodies fused to the UBX domain of the p97 Adapter FAF1. In such a way, we created a p97-directed proteolysis-targeting chimera (PROTAC), representing a unique E3 ubiquitin ligase-independent strategy to promote specific proteolysis.

### Competing Interest Statement

Alejandro Rojas-Fernandez and Constanza Salinas-Rebolledo are inventors of a patent request of the Austral university of Chile.

Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (PROTAC)

p97-단백질-분해-효소를-이용한-특정-단백질-분해-기술


p97 단백질 분해 효소를 이용한 특정 단백질 분해 기술
