인간-로봇-상호작용을-위한-사회적-움직임-예측-기반의-로봇-상호작용-행동-생성

vorhersage-natürlicher-interaktionsbewegungen-für-die-mensch-roboter-interaktion-durch-soziale-bewegungsvorhersage

widespread-and-long-lasting-changes-in-brain-connectivity-induced-by-a-single-exposure-to-drugs-of-abuse-or-anesthetic-agents

学術洞察調査のための階層的ツリー構造型知識グラフ

학술-통찰-조사를-위한-계층적-트리-구조-지식-그래프

hierarchischer-baumstrukturierter-wissensgraph-zur-unterstützung-von-forschungsüberblicksanalysen

traumatic-brain-injury-activates-a-pvr-ap-1-mmp1-signaling-pathway-in-astrocytes

臨床試験参加者マッチングにおける大規模言語モデルの活用


ERK signaling drives the transition from naive to formative pluripotency in mouse embryonic stem cells by both destabilizing the naive state transcription factor network and sustaining the expression of the core pluripotency factor Oct4.


coremsg

Naïve epiblast cells in the embryo and pluripotent stem cells in vitro undergo developmental progression to a formative state competent for lineage specification. During this transition, transcription factors and chromatin are rewired to encode new functional features. Here, we examine the role of mitogen-activated protein kinase (ERK1/2) signalling in pluripotent state transition. We show that a primary consequence of ERK activation in mouse embryonic stem cells is elimination of Nanog, precipitating breakdown of the naïve state gene regulatory network. Cell variability in pERK dynamics results in metachronous down-regulation of Nanog and naïve state exit. Knockdown of Nanog allows exit without ERK activation. However, transition to formative pluripotency does not proceed and cells collapse to an indeterminate identity. This failure is attributable to loss of expression of the central pluripotency factor Oct4. Thus, during formative transition ERK signalling both dismantles the naïve state and preserves pluripotency. These results illustrate that a single signalling pathway can both drive exit from a developmental state and safeguard progression to the successor state.

### Competing Interest Statement

The authors have declared no competing interest.

ERK signalling orchestrates metachronous transition from naïve to formative pluripotency

signaling-pathways-orchestrate-the-transition-from-naive-to-formative-pluripotency-in-mouse-embryonic-stem-cells


Signaling Pathways Orchestrate the Transition from Naive to Formative Pluripotency in Mouse Embryonic Stem Cells


title_rewrite

Regulation of Pluripotency Transition in Mouse Embryonic Stem Cells