
CAD96CA and FGFR1 receptor tyrosine kinases function as cell membrane receptors for insect juvenile hormone, transmitting its signaling for gene expression, calcium increase, and larval development.


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Juvenile hormone (JH) is important to maintain insect larval status; however, its cell membrane receptor has not been identified. Using the lepidopteran insect Helicoverpa armigera (cotton bollworm), a serious agricultural pest, as a model, we determined that receptor tyrosine kinases (RTKs) cadherin 96ca (CAD96CA) and fibroblast growth factor receptor homologue (FGFR1) function as JH cell membrane receptors by their roles in JH-regulated gene expression, larval status maintaining, calcium increase, phosphorylation of JH intracellular receptor MET1 and cofactor Taiman, and high affinity to JH III. Gene knockout of Cad96ca and Fgfr1 by CRISPR/Cas9 in embryo and knockdown in various insect cells, and overexpression of CAD96CA and FGFR1 in mammalian HEK-293T cells all supported CAD96CA and FGFR1 transmitting JH signal as JH cell membrane receptors.

### Competing Interest Statement

The authors have declared no competing interest.

Receptor tyrosine kinases CAD96CA and FGFR1 function as the cell membrane receptors of insect juvenile hormone

receptor-tyrosine-kinases-cad96ca-and-fgfr1-function-as-cell-membrane-receptors-for-insect-juvenile-hormone


Receptor Tyrosine Kinases CAD96CA and FGFR1 Function as Cell Membrane Receptors for Insect Juvenile Hormone
